SRX20007003: WGS of mycoplasma pneumoniae: MDY
1 ILLUMINA (HiSeq X Ten) run: 2.7M spots, 811.7M bases, 298.6Mb downloads
Design: Genomic DNA from 50 mL of the end selected culture and the parent strain was extracted using TIANamp Bacteria DNA Kit (DP302) from Tiangen Biochemical Technology (Beijing) Co., Ltd according to the manufacturers instructions. Genome sequencing was performed by Shanghai Yuanxu Biotechnology Co., Ltd. Briefly, genomic DNA was sheared using KAPA Frag Kit for Enzymatic fragmentation (KK8600, KAPABIOSYSTEMS) and sequencing libraries were constructed via VAHTS Universal DNA Library Prep Kit for Illumina V4 (ND610-02, Vazyme). Pair-end libraries were sequenced on Hiseq X-ten platforms with a read length of 150 bp. The average number of reads per sample was 7,178,870 with more than 200 X coverage on average.
Submitted by: Huashan Hospital of Fudan University
Study:
whole genome sequencing of Mycoplasma pneumoniae mutantsshow Abstracthide Abstractwe performed an in vitro study to select macrolide resistant mutants by five macrolides and characterized the final mutants by whole genome sequencing.
Library:
Name: MDY
Instrument: HiSeq X Ten
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs:
1 run, 2.7M spots, 811.7M bases, 298.6Mb